General Information

  • ID:  hor006242
  • Uniprot ID:  P84385
  • Protein name:  Antimicrobial peptide 5
  • Gene name:  NA
  • Organism:  Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)
  • Family:  Gastrin/cholecystokinin family
  • Source:  animal
  • Expression:  Skin.
  • Disease:  NA
  • Comments:  NA
  • Taxonomy:   Silurana (subgenus), Xenopus (genus), Xenopodinae (subfamily), Pipidae (family), Pipoidea (superfamily), Anura (order), Batrachia (superorder), Amphibia (class), Tetrapoda , Dipnotetrapodomorpha , Sarcopterygii (superclass), Euteleostomi , Teleostomi , Gnathostomata , Vertebrata , Craniata (subphylum), Chordata (phylum), Deuterostomia , Bilateria , Eumetazoa , Metazoa (kingdom), Opisthokonta , Eukaryota (superkingdom),cellular organisms
  • GO MF:  GO:0003674 molecular_function
  • GO BP:  GO:0006952 defense response; GO:0031640 killing of cells of another organism; GO:0042742 defense response to bacterium; GO:0044179 hemolysis in another organism; GO:0050829 defense response to Gram-negative bacterium; GO:0050830 defense response to Gram-positive bacterium; GO:0050832 defense response to fungus
  • GO CC:  GO:0005576 extracellular region

Sequence Information

  • Sequence:  GMATKAGTALGKVAKAVIGAAL
  • Length:  22
  • Propeptide:  GMATKAGTALGKVAKAVIGAAL
  • Signal peptide:  NA
  • Modification:  T22 Leucine amide
  • Glycosylation:  NA
  • Mutagenesis:  NA

Activity

  • Function:  Has very strong antimicrobial activity against Gram-positive bacterium S.aureus, Gram-negative bacterium E.coli and yeast C.albicans. Has strong hemolytic activity against human red blood cells.
  • Mechanism:  NA
  • Cross BBB:  NA
  • Target:  NA
  • Target Unid:  NA
  • IC50: NA
  • EC50: NA
  • ED50: NA
  • kd: NA
  • Half life: NA

Structure

  • Disulfide bond:  NA
  • Structure ID:  AF-P84385-F1(AlphaFold_DB_ID)

  • Structure: (PDB_ID-from https://www.rcsb.org/; AlphaFold_DB_ID-from https://alphafold.ebi.ac.uk/; hordbxxxxxx_AF2.pdb was predicted structure by AlphaFold2; hordbxxxxxx_ESM.pdb was predicted structure by ESMFold)
    •    AF-P84385-F1.pdbhor006242_AF2.pdbhor006242_ESM.pdb

Physical Information

Mass: 237424 Formula: C88H159N25O25S
Absent amino acids: CDEFHNPQRSWY Common amino acids: A
pI: 11.1 Basic residues: 3
Polar residues: 6 Hydrophobic residues: 12
Hydrophobicity: 92.27 Boman Index: 1983
Half-Life: 30 hour Half-Life Yeast: >20 hour
Half-Life E.Coli: >10 hour Aliphatic Index 111.36
Instability Index: -28.18 Extinction Coefficient cystines: 0
Absorbance 280nm: 0

Literature

  • PubMed ID:  11738090
  • Title:  Antimicrobial peptides isolated from skin secretions of the diploid frog, Xenopus tropicalis (Pipidae).